Sampling
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Sampling advice for all conditions covered by the NHS Ayrshire & Arran Empirical Antimicrobial Guidance can be found in the respective organ-system sections of this guide.
Detailed information on sampling requirements and processing (incl. tests sent to other laboratories) is available in the NHS Ayrshire & Arran Laboratory Handbook (desktop access only link).
Empirical treatment relies on more broad spectrum, more toxic agents, or agents associated with a high risk of C. difficile infection, and should not be regarded as a definitive treatment regimen in most cases of infection.
Susceptibility information is only available after culture and allows de-escalation to narrow spectrum, effective alternatives.
Empirical therapy should be reviewed and replaced with a culture-based regimen as soon as results become available.
Good Practice
- The empirical policy only applies in the absence of previous results.
- Sampling of the suspected focus of infection before antimicrobials are started or changed should be undertaken wherever possible.
- Do not obtain blood cultures at insertion of a peripheral venous cannula - this practice is associated with high rates of contamination.
- Samples from sterile sites should be sent to the laboratory in a sterile universal container (white top). If the sample is injected into a blood culture bottle, it is not possible to perform cell counts or microscopy on receipt.
- Samples of pus or fluids in a universal container are preferable to swabs.